• Catalog Number:

  E1A0695A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p130 Cas around the phosphorylation site of tyrosine 165 (D-L-YP-Q-V).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P56945

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  BCA1, BCAR1, Breast cancer anti-estrogen resistance 1 protein, CAS, CRK-associated substrate, CRKAS

• Specificity Sensitivity:

  p130 Cas (Phospho-Tyr165) antibody detects endogenous levels of p130 Cas only when phosphorylated at tyrosine 165.

• References:

  Sara Cabodi, Cancer Res., May 2006; 66: 4672 - 4680. Päivi T. Lakkakorpi, J. Biol. Chem., Feb 1999; 274: 4900. Zhiyuan Zhang, J. Biol. Chem., Aug 1999; 274: 25093. Erguang Li, J. Biol. Chem., May 2000; 275: 14729.

• All Sites:

  Human: Tyr165; Mouse: Tyr169; Rat: Tyr263

Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using p130 Cas (Phospho-Tyr165) antibody.