• Catalog Number:

  E1A0694A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NPM around the phosphorylation site of threonine 199 (R-D-TP-P-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P06748

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 IHC: 1:50~1:100 ELISA: 1:60000

• Other Names:

  B23.1, NPM isoform 1, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, nucleophosmin

• Specificity Sensitivity:

  NPM (Phospho-Thr199) antibody detects endogenous levels of NPM only when phosphorylated at threonine 199.

• References:

  Philipp B Staber, Blood, Aug 2007; 10.1182/blood-2007-02-071258. Brunangelo Falini, Blood, Nov 1999; 94: 3509 - 3515. Jean-François Honorat, Blood, May 2006; 107: 4130 - 4138. Kotaro Funato, Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 291.

• All Sites:

  Human: Thr199; Mouse: Thr198; Rat: Thr198

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using NPM (Phospho-Thr199) antibody. Western blot analysis of extracts from Jurkat cells, using NPM (Phospho-Thr199) antibody.

EGF + - Immunofluorescence analysis of HeLa cells, treated with EGF (200nM, 5mins), using NPM (Phospho-Thr199) antibody.