• Catalog Number:

  E1A0690A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Myosin regulatory light chain 2 around the phosphorylation site of serine 18 (A-T-SP-N-V).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q9CQL8

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  MLCB, MLRA, MLRM, MRCL3, Myosin RLC, Myosin RLC-A, Myosin regulatory light chain, Myosin regulatory light chain 2, nonsarcomeric, Myosin regulatory light chain 2-A, smooth muscle isoform, RLC, RLC-A

• Specificity Sensitivity:

  Myosin regulatory light chain 2 (Phospho-Ser18) antibody detects endogenous levels of Myosin regulatory light chain 2 only when phosphorylated at serine 18.

• References:

  Carninci P., Science 309:1559-1563(2005). The MGC Project Team, Genome Res. 14:2121-2127(2004). Lubec G., Submitted (MAR-2007) to UniProtKB. --------

• All Sites:

  Human: Ser18; Mouse: Ser20; Rat: Ser19

Western blot analysis of extracts from LOVO cells, treated with H2O2 (100uM, 30mins), using Myosin regulatory light chain 2 (Phospho-Ser18) antibody.