• Catalog Number:

  E1A0688A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human mTOR around the phosphorylation site of serine 2481 (I-H-SP-F-I).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P42345

• Applications:

  IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  FRAP, FRAP1, FRAP2, RAFT1, Rapamycin target protein, kinase mTOR

• Specificity Sensitivity:

  mTOR (Phospho-Ser2481) antibody detects endogenous levels of mTOR only when phosphorylated at serine 2481.

• References:

  Hajime Hosoi, Cancer Res., Feb 1999; 59: 886 - 894. P. T. Soliman, ASCO Meeting Abstracts, Jun 2005; 23: 5080. Megan S. Lim, Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 290. Patrick Frost, Blood (ASH Annual Meeting Abstracts), Nov 2004; 104: 646. ---------------------------------------------------------------------------------------------------------------------------------------------------------------

• All Sites:

  Human: Ser2481; Mouse: Ser2481; Rat: Ser2481

P-peptide - +

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human brain tissue using mTOR (Phospho-Ser2481) antibody. Immunofluorescence analysis of NIH/3T3 cells, using mTOR (Phospho-Ser2481) antibody.