• Catalog Number:

  E1A0686A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MSK1 around the phosphorylation site of threonine 581 (L-K-TP-P-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  O75582

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  90 kDa ribosomal protein S6 kinase, , polypeptide 5, EC 2.7.11.1, KS6A5, MSPK1, Nuclear mitogen- and stress-activated protein kinase-1, RLPK, RPS6KA5, kinase MSK1, ribosomal protein S6 kinase, polypeptide V

• Specificity Sensitivity:

  MSK1 (Phospho-Thr581) antibody detects endogenous levels of MSK1 only when phosphorylated at threonine 581.

• References:

  Giselle R. Wiggin, Mol. Cell. Biol., Apr 2002; 22: 2871 - 2881. Greg Q. Butcher, J. Neurosci., Jun 2005; 25: 5305 - 5313. Jason A. Gustin, Am J Physiol Cell Physiol, Mar 2004; 286: C547 - C555. Kyoung Seob Song, J. Biol. Chem., Jun 2003; 278: 23243 - 23250.

• All Sites:

  Human: Thr581; Mouse: Thr645

P-peptide - + Western blot analysis of extracts from RAW264.7 cells, treated with UV (5mins), using MSK1 (Phospho-Thr581) antibody. Immunofluorescence analysis of A549 cells, using MSK1 (Phospho-Thr581) antibody.