Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human MSK1 around the phosphorylation site of serine 360 (T-Y-SP-P-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
O75582
Applications:
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
Other Names:
90 kDa ribosomal protein S6 kinase, , polypeptide 5, EC 2.7.11.1, KS6A5, MSPK1, Nuclear mitogen- and stress-activated protein kinase-1, RLPK, RPS6KA5, kinase MSK1, ribosomal protein S6 kinase, polypeptide V
Specificity Sensitivity:
MSK1 (Phospho-Ser360) antibody detects endogenous levels of MSK1 only when phosphorylated at serine 360.
References:
Deak M., EMBO J. 17:4426-4441(1998).
New L., J. Biol. Chem. 274:1026-1032(1999).
Zhang Y., J. Biol. Chem. 279:21866-21872(2004).
All Sites:
Human: Ser360; Mouse: Ser359
P-peptide - +
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MSK1 (Phospho-Ser360) antibody.
Immunofluorescence analysis of NIH/3T3 cells, using MSK1 (Phospho-Ser360) antibody.