• Catalog Number:

  E1A0681A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of threonine 286 (A-E-TP-P-P).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q02750

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  Dual specificity mitogen-activated protein kinase kinase 1, EC 2.7.12.2, ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK1, MP2K1, PRKMK1, kinase MEK1

• Specificity Sensitivity:

  MEK1 (Phospho-Thr286) antibody detects endogenous levels of MEK1 only when phosphorylated at threonine 286.

• References:

  Shunichi Murakami, Genes & Dev., Feb 2004; 18: 290. Ronald A. Rimerman, J. Biol. Chem., May 2000; 275: 14736. Anne C. Marinovic, J. Biol. Chem., May 2002; 277: 16673 - 16681. Rohit Lal, AACR Meeting Abstracts, Apr 2006; 2006: 687.

• All Sites:

  Human: Thr286; Mouse: Thr286; Rat: Thr286

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (Phospho-Thr286) antibody. Western blot analysis of extracts from K562 cells, using MEK1 (Phospho-Thr286) antibody.