• Catalog Number:

  E1A0677A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MAPKAPK2 around the phosphorylation site of threonine 222 (L-T-TP-P-C).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P49137

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  EC 2.7.11.1; kinase MAPKAPK2; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPK2; MAPKAP kinase 2; MAPKAPK-2; MAPKAPK2; RPS6KC1

• Specificity Sensitivity:

  MAPKAPK2 (Phospho-Thr222) antibody detects endogenous levels of MAPKAPK2 only when phosphorylated at threonine 222.

• References:

  Zu Y.-L., Biochem. Biophys. Res. Commun. 200:1118-1124(1994). The MGC Project Team; Genome Res. 14:2121-2127(2004). Stokoe D., Biochem. J. 296:843-849(1993).

• All Sites:

  Human: Thr222; Mouse: Thr222; Rat: Thr222

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human heart tissue using MAPKAPK2 (Phospho-Thr222) antibody. Western blot analysis of extracts from COS-7 cells treated and untreated with UV (15mins), using MAPKAPK2 (Phospho-Thr222) antibody.