Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human LKB1 around the phosphorylation site of throsine 189 (G-G-TP-L-K).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
Q15831
Applications:
WB: 1:500~1:1000 ELISA: 1:1000
Other Names:
DBCN, DCX, Doublin, LISX, Lis-X, Lissencephalin-X
Specificity Sensitivity:
LKB1 (Phospho-Thr189) antibody detects endogenous levels of LKB1 only when phosphorylated at throsine 189.
References:
Nezu J.; Nat. Genet. 18:38-43(1998). Bignell G.R., Cancer Res. 58:1384-1386(1998).
Scanlan M.J., Int. J. Cancer 83:456-464(1999).
Baas A.F., EMBO J. 22:3062-3072(2003).
All Sites:
Human: Thr189; Mouse: Thr189
Western blot analysis of extracts from NIH/3T3 cells, treated with PMA (125ng/ml, 30mins), using LKB1 (Phospho-Thr189) antibody.