• Catalog Number:

  E1A0669A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human LAT around the phosphorylation site of tyrosine 171 (D-D-YP-V-N).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  O43561 

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  36 kDa phospho-tyrosine adaptor protein; Linker for activation of T cells; p36-38; pp36

• Specificity Sensitivity:

  LAT (Phospho-Tyr171) antibody detects endogenous levels of LAT only when phosphorylated at tyrosine 171

• References:

  M Nicosia, J. Virol., Dec 1993; 67: 7276 - 7283. Marta Pineda, J. Biol. Chem., Jul 1999; 274: 19738. Shin-ichiroh Saitoh, J. Exp. Med., Sep 2003; 198: 831.

• All Sites:

  Human: Tyr171; Mouse: Tyr171

Western blot analysis of extracts from Jurkat cells, treated with UV (5mins), using LAT (Phospho-Tyr171) antibody.