Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 794 (S-T-SP-S-G).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P35568
Applications:
IHC: 1:50~1:100 ELISA: 1:40000
Other Names:
IRS-1, IRS1, Insulin receptor substrate-1
Specificity Sensitivity:
IRS-1 (Phospho-Ser794) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 794.
References:
Joëlle Dupont, Am J Physiol Endocrinol Metab, Feb 1998; 274: E309 - E316.
DG Baskin, Endocrinology, Apr 1994; 134: 1952.
Zhefu Ma, Mol. Cell. Biol., Dec 2006; 26: 9338 - 9351.
Alexandros Tzatsos, J. Biol. Chem., Jun 2007; 282: 18069 - 18082.
All Sites:
Human: Ser794; Mouse: Ser789; Rat: Ser789
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using IRS-1 (Phospho-Ser794) antibody.