• Catalog Number:

  E1A0662A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Histone H3 around the phosphorylation site of threonine 11 (K-S-TP-G-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P68431

• Applications:

  IF: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  H3/a, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, H31, HIST1H3A, histone H3.1

• Specificity Sensitivity:

  Histone H3 (Phospho-Thr11) antibody detects endogenous levels of Histone H3 only when phosphorylated at threonine 11.

• References:

  Kyu Heo. J. Biol. Chem., May 2007; 282: 15476 - 15483. Sandra B. Hake. PNAS, Apr 2006; 103: 6428 - 6435. Jeong-Heon Lee. J. Biol. Chem., May 2007; 282: 13419 - 13428.

• All Sites:

  Human: Thr11; Mouse: Thr11; Rat: Thr12

P-peptide - +

Immunofluorescence analysis of HuvEc cells, using Histone H3 (Phospho-Thr11) antibody. Histone H3 (Phospho-Thr11) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.