• Catalog Number:

  E1A0641A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human eIF4B around the phosphorylation site of serine 422 (T-G-SP-E-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P23588

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  Eukaryotic translation initiation factor 4B, IF4B, eIF-4B

• Specificity Sensitivity:

  eIF4B (Phospho-Ser422) antibody detects endogenous levels of eIF4B only when phosphorylated at serine 422.

• References:

  S. Lopez de Quinto. RNA, Sep 2001; 7: 1213. Rosalyn C. Doepker. J. Virol., May 2004; 78: 4684 - 4699. Pinghui Feng. J. Virol., Aug 2005; 79: 9651 - 9664. Kerstin Ochs. J. Virol., Jan 2003; 77: 115 - 122.

• All Sites:

  Human: Ser422; Mouse: Ser422; Rat: Ser422

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using eIF4B (Phospho-Ser422) antibody. Western blot analysis of extracts from NIH/3T3cells, using eIF4B (Phospho-Ser422) antibody.

P-peptide - + Immunofluorescence analysis of NIH/3T3 cells, using eIF4B (Phospho-Ser422) antibody.