• Catalog Number:

  E1A0636A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Cyclin E1 around the phosphorylation site of threonine 77 (I-P-TP-P-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P24864

• Applications:

  IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  CCNE, G1/S-specific cyclin E1, cyclin E1

• Specificity Sensitivity:

  Cyclin E1 (Phospho-Thr77) antibody detects endogenous levels of Cyclin E1 only when phosphorylated at threonine 77.

• References:

  Koff A., Cell 66:1217-1228(1991). The MGC Project Team, Genome Res. 14:2121-2127(2004). Geng Y., Oncogene 12:1173-1180(1996).

• All Sites:

  Human: Thr77; Mouse: Thr74

P-peptide - + Immunofluorescence analysis of HeLa cells, using Cyclin E1 (Phospho-Thr77) antibody.