• Catalog Number:

  E1A0634A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human c-Raf around the phosphorylation site of serine 296 (S-S-SP-P-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P04049

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  C-RAF, C-Raf, CRAF, EC 2.7.11.1, Protein kinase raf 1, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1, Raf proto-oncogene serine/threonine-protein kinase, Raf-1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, kinase Raf1

• Specificity Sensitivity:

  c-Raf (Phospho-Ser296) antibody detects endogenous levels of c-Raf only when phosphorylated at serine 296.

• References:

  Marie A. Bogoyevitch, J. Biol. Chem., Nov 1995; 270: 26303. G Heidecker, Mol. Cell. Biol., Jun 1990; 10: 2503 - 2512. Shari L. Sutor, J. Biol. Chem., Mar 1999; 274: 7002. Albrecht Piiper, Mol. Pharmacol., Sep 2000; 58: 608. -------------------------------------------------------------------------------------------------------------------------------------------------------- --------

• All Sites:

  Human: Ser296; Mouse: Ser296; Rat: Ser296

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using c-Raf (Phospho-Ser296) antibody. Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using c-Raf (Phospho-Ser296) antibody.