• Catalog Number:

  E1A0629A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 296 (I-Q-SP-N-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  O14757

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  CHEK1; Chk1; EC 2.7.11.1; kinase Chk1

• Specificity Sensitivity:

  Chk1 (Phospho-Ser296) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 296.

• References:

  Sanchez Y., Science 277:1497-1501(1997). Flaggs G., Curr. Biol. 7:977-986(1997). Semba S., Int. J. Oncol. 16:731-737(2000).

• All Sites:

  Human: Ser296

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Chk1 (Phospho-Ser296) antibody. Western blot analysis of extracts from HUVEC cells, treated with UV (15mins), using Chk1 (Phospho-Ser296) antibody.