• Catalog Number:

  E1A0628A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CDC25C around the phosphorylation site of threonine 48 (P-R-TP-P-V).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P30307

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  CDC25M1, Dual specificity phosphatase Cdc25C, EC 3.1.3.48, M-phase inducer phosphatase 3, MPIP3

• Specificity Sensitivity:

  CDC25C (Phospho-Thr48) antibody detects endogenous levels of CDC25C only when phosphorylated at threonine 48.

• References:

  Bureik M., Int. J. Oncol. 17:1251-1258(2000). Hernandez S., Lab. Invest. 81:465-473(2001). Beausoleil S.A., Nat. Biotechnol. 24:1285-1292(2006).

• All Sites:

  Human: Thr48; Mouse: Thr48

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using CDC25C (Phospho-Thr48) antibody. Western blot analysis of extracts from RAW264.7 cells, using CDC25C (Phospho-Thr48) antibody.