• Catalog Number:

  E1A0626A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CD32around the phosphorylation site of tyrosine 292 (I-T-YP-S-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P31994 

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  CD32, CDW32, FC gamma receptor IIB, FC-gamma RII, FC-gamma RII-B, FC-gamma-RIIB, FCG2, FCG2B, FCGR2, FCGR2B, FCRII, FCgammaRIIB, IGFR2, IGG FC receptor II beta, IGG FC receptor II-B, LY-17, Low affinity immunoglobulin gamma FC region receptor II precursor, Low affinity immunoglobulin gamma FC region receptor II-B precursor

• Specificity Sensitivity:

  CD32 (Phospho-Tyr292) antibody detects endogenous levels of CD32 only when phosphorylated at tyrosine 292.

• References:

  Stuart S.G., EMBO J. 8:3657-3666(1989). Brooks D.G., J. Exp. Med. 170:1369-1385(1989). Engelhardt W., Eur. J. Immunol. 20:1367-1377(1990).

• All Sites:

  Human: Tyr292

Western blot analysis of extracts from K562 cells, treated with PMA (125ng/ml, 30mins), using CD32 (Phospho-Tyr292) antibody.