• Catalog Number:

  E1A0624A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human c-Abl around the phosphorylation site of tyrosine 245 (T-V-YP-G-V).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P00519

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  ABL1, EC 2.7.1.112, JTK7, Proto-oncogene tyrosine-protein kinase ABL1, c-ABL, p150

• Specificity Sensitivity:

  c-Abl (Phospho-Tyr245) antibody detects endogenous levels of c-Abl only when phosphorylated at tyrosine 245.

• References:

  Fainstein E., Oncogene 4:1477-1481(1989). Chissoe S.L., Genomics 27:67-82(1995). Olsen J.V., Cell 127:635-648(2006). ’

• All Sites:

  Human: Tyr245; Mouse: Tyr245; Rat: Tyr245

Western blot analysis of extracts from K562 cells, treated with Insulin (0.01U/ml, 15mins), using c-Abl (Phospho-Tyr245) antibody.