Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human BLNK around the phosphorylation site of tyrosine 96 (D-S-YP-E-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
Q8WV28
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Other Names:
B-cell linker protein, LY57, SLP-65, SLP65
Specificity Sensitivity:
BLNK (Phospho-Tyr96) antibody detects endogenous levels of BLNK only when phosphorylated at tyrosine 96.
References:
Francisco A. Bonilla. PNAS, Feb 2000; 97: 1725.
Rajita Pappu. Science, Dec 1999; 286: 1949.
Joy En-Lin Tan. J. Biol. Chem., Jun 2001; 276: 20055 - 20063.
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All Sites:
Human: Tyr96; Mouse: Tyr96
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human lymph node tissue using BLNK (Phospho-Tyr96) antibody.
Western blot analysis of extracts from COLO205 cells, using BLNK (Phospho-Tyr96) antibody.