• Catalog Number:

  E1A0609A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of serine 129 (D-N-SP-G-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P31749

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  AKT; AKT1 kinase; C-AKT; EC 2.7.11.1; kinase Akt1; PKB; PKB-alpha; Protein kinase B; RAC; RAC-alpha serine/threonine kinase; RAC-PK-alpha

• Specificity Sensitivity:

  Akt (Phospho-Ser129) antibody detects endogenous levels of Akt only when phosphorylated at serine 129.

• References:

  Jones P.F., Proc. Natl. Acad. Sci. U.S.A. 88:4171-4175(1991). Matsubara A., Diabetologia 44:910-913(2001). The MGC Project Team; Genome Res. 14-2121-2127(2004).

• All Sites:

  Human: Ser129; Mouse: Ser129; Rat: Ser129

Western blot analysis of extracts from A549 cells, treated with PMA (125ng/ml, 30mins), using Akt (Phospho-Ser129) antibody.