• Catalog Number:

  E1A0604A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ACK1 around the phosphorylation site of tyrosine 284 (D-H-YP-V-M).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q07912

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  ACK1, Activated p21cdc42Hs kinase, EC 2.7.10.2, Non-receptor protein tyrosine kinase Ack, TNK2, kinase ACK1

• Specificity Sensitivity:

  ACK1 (Phospho-Tyr284) antibody detects endogenous levels of ACK1 only when phosphorylated at tyrosine 284.

• References:

  Manser E., Nature 363:364-367(1993). Ota T., Nat. Genet. 36:40-45(2004). Eisenmann K.M., Nat. Cell Biol. 1:507-513(1999).

• All Sites:

  Human: Tyr284; Mouse: Tyr284

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody. Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using ACK1 (Phospho-Tyr284) antibody.

P-peptide - + Immunofluorescence analysis of A549 cells, using ACK1 (Phospho-Tyr284) antibody.