• Catalog Number:

  E1A0595A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human VEGFR2 around the phosphorylation site of tyrosine 1059(P-D-YP-V-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35968

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  EC 2.7.10.1, FLK1, KDR, VGFR2, VGR2, kinase insert domain receptor, protein-tyrosine kinase receptor Flk-1, vascular endothelial growth factor receptor 2

• Specificity Sensitivity:

  VEGFR2 (Phospho-Tyr1059) antibody detects endogenous levels of VEGFR2 only when phosphorylated at tyrosine 1059.

• References:

  Terman B.I., Oncogene 6:1677-1683(1991). Patterson C., J. Biol. Chem. 270:23111-23118(1995). Mitola S., J. Virol. 74:344-353(2000).

• All Sites:

  Human: Tyr1059; Mouse: Tyr1057; Rat: Tyr1055

Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3nM, 40mins), using VEGFR2 (Phospho-Tyr1059) antibody.