• Catalog Number:

  E1A0594A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human VEGFR1 around the phosphorylation site of tyrosine 1333 (V-L-YP-S-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P17948

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  EC 2.7.1.112, EC 2.7.10.1, FLT, FLT1, FRT, Flt-1, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, VGFR1, Vascular endothelial growth factor receptor 1 precursor, Vascular permeability factor receptor

• Specificity Sensitivity:

  VEGFR1 (Phospho-Tyr1333) antibody detects endogenous levels of VEGFR1 only when phosphorylated at tyrosine 1333.

• References:

  Shibuya M., Oncogene 5:519-524(1990). Kendall R.L., Proc. Natl. Acad. Sci. U.S.A. 90:10705-10709(1993). The MGC Project Team, Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Tyr1333; Mouse: Tyr1328; Rat: Tyr1331

Western blot analysis of extracts from K562 cells, treated with etoposide (25uM, 24hours), using VEGFR1 (Phospho-Tyr1333) antibody.