Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Tuberin/TSC2 around the phosphorylation site of threonine 1462 (G-Y-TP-I-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P49815
Applications:
IHC: 1:50~1:100 ELISA: 1:1000
Other Names:
TSC2, Tuberous sclerosis 2 homolog protein, Tuberous sclerosis 2 protein
Specificity Sensitivity:
Tuberin/TSC2 (Phospho-Thr1462) antibody detects endogenous levels of Tuberin/TSC2 only when phosphorylated at threonine 1462.
References:
DL Levey, J. Exp. Med., Oct 1995; 182: 1029.
Liguang Chen, Blood, Mar 2005; 105: 2036 - 2041.
Adrian Wiestner, Blood, Jun 2003; 101: 4944 - 4951.
Makoto Otsu, Blood, Jul 2002; 100: 1248 - 1256.
All Sites:
Human: Thr1462; Mouse: Thr1465; Rat: Thr1466
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Tuberin/TSC2 (Phospho-Thr1462) antibody.