• Catalog Number:

  E1A0589A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Trk A around the phosphorylation site of tyrosine 496 (P-Q-YP-F-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P04629

• Applications:

  IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  EC 2.7.10.1, High affinity nerve growth factor receptor precursor, NTRK1, Slow nerve growth factor receptor, TRK, TRK1 transforming tyrosine kinase protein, TRKA, Trk-A, kinase TrkA, p140-TrkA

• Specificity Sensitivity:

  Trk A (Phospho-Tyr496) antibody detects endogenous levels of Trk A only when phosphorylated at tyrosine 496.

• References:

  E Lavenius. Cell Growth Differ., Jun 1995; 6: 727. F Miralles. J. Endocrinol., Mar 1998; 156: 431 - 439. Tim R. Bilderback. J. Biol. Chem., Jan 1999; 274: 257.

• All Sites:

  Human: Tyr496; Mouse: Tyr499; Rat: Tyr499

P-peptide - +

Immunofluorescence analysis of NIH/3T3 cells, using Trk A (Phospho-Tyr496) antibody. Trk A (Phospho-Tyr496) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.