• Catalog Number:

  E1A0587A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human TNNI3 around the phosphorylation site of serine 22 (R-R-SP-SP-A-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P19429

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  TNNC1, troponin I, cardiac muscle

• Specificity Sensitivity:

  TNNI3 (Phospho-Ser22+Ser23) antibody detects endogenous levels of TNNI3 only when phosphorylated at serine 22.

• References:

  Raffaella Di Lisi, J. Biol. Chem., Sep 1998; 273: 25371. Emile Missov, Circulation, Nov 1997; 96: 2953 - 2958. Brandon J. Biesiadecki, J. Biol. Chem., Apr 2004; 279: 13825 - 13832.

• All Sites:

  Human: Ser22+Ser23; Mouse: Ser22+Ser23; Rat: Ser22+Ser23

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using TNNI3 (Phospho-Ser22+Ser23) antibody. Western blot analysis of extracts from mouse heart cells, using TNNI3 (Phospho-Ser22+Ser23) antibody.