• Catalog Number:

  E1A0586A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human TK around the phosphorylation site of serine 13 (P-G-SP-P-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P04183

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  EC 2.7.1.21, KITH, TK-1, TK1, Thymidine kinase, cytosolic

• Specificity Sensitivity:

  TK (Phospho-Ser13) antibody detects endogenous levels of TK only when phosphorylated at serine 13.

• References:

  N Singh, Mol. Cell. Biol., May 1991; 11: 2362 - 2374. Bin Tian, Am. J. Pathol., Aug 2003; 163: 789. René Gerolami, Cancer Res., Feb 2000; 60: 993 - 1001. Catia Traversari, Blood, Jun 2007; 109: 4708 - 4715.

• All Sites:

  Human: Ser13; Mouse: Ser13

Western blot analysis of extracts from HeLa cells, treated with paclitaxel (1uM, 24hours), using TK (Phospho-Ser13) antibody.