• Catalog Number:

  E1A0584A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human TFII-I around the phosphorylation site of tyrosine 248 (P-D-YP-Y-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P78347

• Applications:

  WB: 1:500~1:1000 ELISA: 1:500

• Other Names:

  BAP-135, BAP135, BTK-associated protein-135, Bruton tyrosine kinase-associated protein-135, DIWS1T, GTF2I, GTFI, GTFII-I, General II-I, General transcription factor II-I, SPIN, SRF-Phox1 interacting protein, TFII-I, WBSCR6, Williams-Beuren syndrome chromosome region 6 protein

• Specificity Sensitivity:

  TFII-I (Phospho-Tyr248) antibody detects endogenous levels of TFII-I only when phosphorylated at tyrosine 248.

• References:

  Roy A.L., EMBO J. 16:7091-7104(1997). Grueneberg D.A., Genes Dev. 11:2482-2493(1997). Yang W., Proc. Natl. Acad. Sci. U.S.A. 94:604-609(1997).

• All Sites:

  Human: Tyr248; Mouse: Tyr248; Rat: Tyr248

Western blot analysis of extracts from LOVO cells, using TFII-I (Phospho-Tyr248) antibody.