• Catalog Number:

  E1A0580A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SYK around the phosphorylation site of tyrosine 323 (N-P-YP-E-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P43405

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  EC 2.7.10.2, KSYK, SYK, Spleen tyrosine kinase, kinase Syk

• Specificity Sensitivity:

  SYK (Phospho-Tyr323) antibody detects endogenous levels of SYK only when phosphorylated at tyrosine 323.

• References:

  Yagi S., Biochem. Biophys. Res. Commun. 200:28-34(1994). The MGC Project Team, ome Res. 14:2121-2127(2004). Salomon A.R., Proc. Natl. Acad. Sci. U.S.A. 100:443-448(2003).

• All Sites:

  Human: Ser323; Mouse: Ser317; Rat: Ser317

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using SYK (Phospho-Tyr323) antibody. Western blot analysis of extracts from HT-29 cells, using SYK (Phospho-Tyr323) antibody.