• Catalog Number:

  E1A0578A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Survivin around the phosphorylation site of threonine 34 (A-C-TP-P-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  O15392

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  API4; apoptosis inhibitor 4; apoptosis inhibitor survivin; baculoviral IAP repeat-containing protein 5; BIRC5; IAP4; TIAP

• Specificity Sensitivity:

  Survivin (Phospho-Thr34) antibody detects endogenous levels of Survivin only when phosphorylated at threonine 34.

• References:

  Ambrosini G., Nat. Med. 3:917-921(1997). Mahotka C., Cancer Res. 59:6097-6102(1999). Uren A.G., Curr. Biol. 10:1319-1328(2000).

• All Sites:

  Human: Thr34; Mouse: Thr34

Western blot analysis of extracts from HT-29 cells, treated with EGF (100μg/ml, 30mins), using Survivin (Phospho-Thr34) antibody.