• Catalog Number:

  E1A0577A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SP1 around the phosphorylation site of threonine 453 (I-R-TP-P-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P08047

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  TSFP1, Transcription factor SP1, transcription factor Sp1

• Specificity Sensitivity:

  SP1 (Phospho-Thr453) antibody detects endogenous levels of SP1 only when phosphorylated at threonine 453.

• References:

  Zhenjun Lou, Cancer Res., Feb 2005; 65: 1007 - 1017. Kolaparthi Venkatasubbarao, Cancer Res., Aug 2001; 61: 6239 - 6247. George Koutsodontis, J. Biol. Chem., Jul 2001; 276: 29116 - 29125. Shi-Hua Li, Mol. Cell. Biol., Mar 2002; 22: 1277 - 1287.

• All Sites:

  Human: Thr453; Mouse: Thr455; Rat: Thr456

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using SP1 (Phospho-Thr453) antibody. Western blot analysis of extracts from A549 cells, using SP1 (Phospho-Thr453) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using SP1 (Phospho-Thr453) antibody.