• Catalog Number:

  E1A0576A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SOX-9 around the phosphorylation site of serine 181 (R-K-SP-V-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P48436

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  SRY sex determining region Y-box 9 protein, SRY-box containing gene 9, Sox-9, transcription factor SOX-9

• Specificity Sensitivity:

  SOX-9 (Phospho-Ser181) antibody detects endogenous levels of SOX-9 only when phosphorylated at serine 181.

• References:

  E. Kolettas, Rheumatology, Oct 2001; 40: 1146 - 1156. Céline J. Guigon, Endocrinology, Jul 2005; 146: 2992 - 3004. Andrea S. Cupp, Biol Reprod, Jun 2003; 68: 2033. Sang-Gu Hwang, J. Biol. Chem., Aug 2005; 280: 29780 - 29787.

• All Sites:

  Human: Ser181; Mouse: Ser181

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using SOX-9 (Phospho-Ser181) antibody. Western blot analysis of extracts from 293 cells, treated with PBS (60mins), using SOX-9 (Phospho-Ser181) antibody.

P-peptide - + Immunofluorescence analysis of A549 cells, using SOX-9 (Phospho-Ser181) antibody.