• Catalog Number:

  E1A0572A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SF1 around the phosphorylation site of serine 82 (S-P-SP-P-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q15637

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  BBP, CW17, Mammalian branch point binding protein mBBP, SF01, Splicing factor 1, Splicing factor SF1, Transcription factor ZFM1, ZFM1, ZFP162, ZNF162, Zinc finger gene in MEN1 locus, Zinc finger protein 162, Mzfm

• Specificity Sensitivity:

  SF1 (Phospho-Ser82) antibody detects endogenous levels of SF1 only when phosphorylated at serine 82.

• References:

  Susan Y. Park, Development, May 2005; 132: 2415 - 2423. Dujin Zhou, Mol. Endocrinol., Jul 2000; 14: 986. Dagmar Wilhelm, Genes & Dev., Jul 2002; 16: 1839. Philipp Gut,Development, Oct 2005; 132: 4611 - 4619.

• All Sites:

  Human: Ser82; Mouse: Ser82

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using SF1 (Phospho-Ser82) antibody. Western blot analysis of extracts from HUVEC cells, treated with anisomycin (25ug/ml, 30mins), using SF1 (Phospho-Ser82) antibody.

P-peptide - + Immunofluorescence analysis of A549 cells, using SF1 (Phospho-Ser82) antibody.