• Catalog Number:

  E1A0571A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human S6 Ribosomal Protein around the phosphorylation site of serine 240 (R-A-SP-T-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P62753

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  40S ribosomal protein S6, Phosphoprotein NP33, RPS6, RS6, ribosomal protein S6

• Specificity Sensitivity:

  S6 Ribosomal Protein (Phospho-Ser240) antibody detects endogenous levels of S6 Ribosomal Protein only when phosphorylated at serine 240.

• References:

  J Papkoff, Mol. Cell. Biol., Jan 1994; 14: 463 - 472. Kellie L. Watson, PNAS, Nov 1996; 93: 13694. Rebecca Cruz, J. Leukoc. Biol., Dec 2005; 78: 1378 - 1385. Yoshinori Seko, Circ. Res., Jan 1996; 78: 82 - 90.

• All Sites:

  Human: Ser240; Mouse: Ser240; Rat: Ser240

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using S6 Ribosomal Protein (Phospho-Ser240) antibody. Western blot analysis of extracts from HeLa cells, treated with TNF-a (20ng/ml, 2mins), using S6 Ribosomal Protein (Phospho-Ser240) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using S6 Ribosomal Protein (Phospho-Ser240) antibody.