Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human RyR2 around the phosphorylation site of serine 2808 (R-I-SP-Q-T).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
RyR2 (Phospho-Ser2808) antibody detects endogenous levels of RyR2 only when phosphorylated at serine 2808.
References:
Dawei Jiang, Circ. Res., Nov 2005; 97: 1173 - 1181.
Guojin Huang, J. Biol. Chem., May 2007; 282: 14283 - 14290.
James D. Johnson, J. Biol. Chem., Jun 2004; 279: 24794 - 24802.
Guangju Ji, J. Gen. Physiol., Mar 2004; 123: 377 - 386.
All Sites:
Human: Ser2808; Mouse: Ser2808
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using RyR2 (Phospho-Ser2808) antibody.