Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Rhodopsin around the phosphorylation site of serine 334 (D-A-SP-A-T).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P08100
Applications:
IHC: 1:50~1:100 ELISA: 1:1000
Other Names:
OPN2, OPSD, RHO, opsin 2
Specificity Sensitivity:
Rhodopsin (Phospho-Ser334) antibody detects endogenous levels of Rhodopsin only when phosphorylated at serine 334.
References:
Edwin S. Lee, Invest. Ophthalmol. Vis. Sci., Jun 2007; 48: 2868 - 2876.
DJ Roof, Invest. Ophthalmol. Vis. Sci., Nov 1994; 35: 4049.
Angelika Schmitt, J. Exp. Biol., Apr 2005; 208: 1247 - 1256.
Paul A. Sieving, J. Neurosci., Aug 2001; 21: 5449.
All Sites:
Human: Ser334; Mouse: Ser334; Rat: Ser334
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Rhodopsin (Phospho-Ser334) antibody.