• Catalog Number:

  E1A0567A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Ret around the phosphorylation site of tyrosine 1015 (R-D-YP-L-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P07949

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  C-ret; EC 2.7.10.1; kinase Ret; Proto-oncogene ret precursor

• Specificity Sensitivity:

  Ret (Phospho-Tyr1015) antibody detects endogenous levels of Ret only when phosphorylated at tyrosine 1015.

• References:

  Takahashi M.; Oncogene 4:805-806(1989). Takahashi M., Oncogene 3:571-578(1988). Takahashi M., Mol. Cell. Biol. 7:1378-1385(1987).

• All Sites:

  Human: Tyr1015; Mouse: Tyr1016; Rat: Tyr1037

Western blot analysis of extracts from COS-7 cells, treated with EGF (200ng/ml, 30mins), using Ret (Phospho-Tyr1015) antibody.