• Catalog Number:

  E1A0566A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human RAF1 around the phosphorylation site of serine 621 (S-A-SP-E-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P04049

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  C-RAF, C-Raf, CRAF, EC 2.7.11.1, Protein kinase raf 1, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1, Raf proto-oncogene serine/threonine-protein kinase, Raf-1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, kinase Raf1

• Specificity Sensitivity:

  Raf1 (Phospho-Ser621) antibody detects endogenous levels of RAF1 only when phosphorylated at serine 621.

• References:

  David R. Hodge, J. Biol. Chem., Jun 1998; 273: 15727. Michael A. Beazely, Mol. Pharmacol., Jan 2005; 67: 250 - 259. Deanna G. Adams, J. Biol. Chem., Dec 2005; 280: 42644 - 42654.

• All Sites:

  Human: Ser621; Mouse: Ser621; Rat: Ser621

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Raf1 (Phospho-Ser621) antibody. Western blot analysis of extracts from HeLa cells, treated with UV (5mins), using Raf1 (Phospho-Ser621) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using Raf1 (Phospho-Ser621) antibody.