• Catalog Number:

  E1A0563A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Rabphilin 3A around the phosphorylation site of serine 237 (R-A-SP-E-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P47708

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  RP3A, RPH3A, exophilin 1

• Specificity Sensitivity:

  Rabphilin 3A (Phospho-Ser237) antibody detects endogenous levels of Rabphilin 3A only when phosphorylated at serine 237.

• References:

  H Shirataki, Mol. Cell. Biol., Apr 1993; 13: 2061 - 2068. N Masumoto, J. Cell Biol., Dec 1996; 135: 1741. M.E. Burns, J. Gen. Physiol., Feb 1998; 111: 243. EM Fykse, J. Neurosci., Mar 1995; 15: 2385.

• All Sites:

  Human: Ser237; Mouse: Ser234; Rat: Ser234

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Rabphilin 3A (Phospho-Ser237) antibody. Western blot analysis of extracts from Raw264.7 cells, treated with Calyculin (100nM, 15mins), using Rabphilin 3A (Phospho-Ser237) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using Rabphilin 3A (Phospho-Ser237) antibody.