Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PTPRA around the phosphorylation site of tyrosine 798 (S-D-YP-A-N).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
PTPRA (Phospho-Tyr798) antibody detects endogenous levels of PTPRA only when phosphorylated at tyrosine 798.
References:
Sap J., Proc. Natl. Acad. Sci. U.S.A. 87:6112-6116(1990).
Kaplan R., Proc. Natl. Acad. Sci. U.S.A. 87:7000-7004(1990).
Krueger N.X., EMBO J. 9:3241-3252(1990).
All Sites:
Human: Tyr798; Mouse: Tyr825; Rat: Tyr792
Western blot analysis of extracts from HeLa cells, treated with serum (20%, 15mins), using PTPRA (Phospho-Tyr798) antibody.