Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PTEN around the phosphorylation site of serine 385 (T-D-SP-D-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
PTEN (Phospho-Ser385) antibody detects endogenous levels of PTEN only when phosphorylated at serine 385.
References:
Graham Jones, Cancer Res., Aug 2001; 61: 5688 - 5691.
Jill M. Stahl, Cancer Res., Jun 2003; 63: 2881 - 2890.
Liang-Ping Weng, Hum. Mol. Genet., Feb 2001; 10: 251 - 258.
Tatsuya Abe, Cancer Res., May 2003; 63: 2300 - 2305.
All Sites:
Human: Ser385; Mouse: Ser385
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using PTEN (Phospho-Ser385) antibody.