• Catalog Number:

  E1A0559A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PR around the phosphorylation site of serine 400 (A-R-SP-P-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P06401

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  NR3C3, PGR, PRGR, Progesterone receptor

• Specificity Sensitivity:

  PR (Phospho-Ser400) antibody detects endogenous levels of PR only when phosphorylated at serine 400.

• References:

  Christiane Nawrath, PLANT CELL, Aug 1999; 11: 1393. Britta M. Jacobsen, Mol. Endocrinol., Mar 2005; 19: 574 - 587. Joseph D. Clarke, PLANT CELL, Apr 1998; 10: 557. Jonathan J. Li, Endocrinology, Sep 2001; 142: 4006.

• All Sites:

  Human: Ser400; Mouse: Ser398

P-peptide - + Western blot analysis of extracts from 293 cells, treated with heat shock, using PR (Phospho-Ser400) antibody. Immunofluorescence analysis of A549 cells, using PR (Phospho-Ser400) antibody.