• Catalog Number:

  E1A0558A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PR around the phosphorylation site of serine 294 (G-R-SP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P06401

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  NR3C3, PGR, PRGR, Progesterone receptor

• Specificity Sensitivity:

  PR (Phospho-Ser294) antibody detects endogenous levels of PR only when phosphorylated at serine 294.

• References:

  Kastner P., EMBO J. 9:1603-1614(1990). Knotts T.A., J. Biol. Chem. 276:8475-8483(2001). Hsiao P.W., Mol. Cell. Biol. 23:6210-6220(2003).

• All Sites:

  Human: Ser294; Mouse: Ser294

P-peptide - + Western blot analysis of extracts from Jurkat cells, treated with Etoposide (25uM, 60mins), using PR (Phospho-Ser294) antibody. Immunofluorescence analysis of HeLa cells, using PR (Phospho-Ser294) antibody.