• Catalog Number:

  E1A0557A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PPAR-γ around the phosphorylation site of serine 112 (P-A-SP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P37231

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  NR1C3, PPAR-gamma-1, Peroxisome proliferator activated receptor gamma

• Specificity Sensitivity:

  PPAR-γ (Phospho-Ser112) antibody detects endogenous levels of PPAR-γ only when phosphorylated at serine 112.

• References:

  Filiz Akbiyik, Blood, Sep 2004; 104: 1361 - 1368. Robert B. Clark, J. Immunol., Feb 2000; 164: 1364. JOSUÉ PADILLA, Ann. N.Y. Acad. Sci., Apr 2000; 905: 97. Chinyu G. Su, J. Clin. Invest., Aug 1999; 104: 383.

• All Sites:

  Human: Ser112; Mouse: Ser112

Western blot analysis of extracts from Jurkat cells, treated with Paclitaxel (1uM, 24hours), using PPAR-γ (Phospho-Ser112) antibody.