• Catalog Number:

  E1A0556A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PPAR-β around the phosphorylation site of threonine 1457 (A-Y-TP-P-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q15648

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  ARC205, Activator-recruited cofactor 205 kDa component, DRIP205, PBP, PPAR-binding protein, PPRB, Peroxisome proliferator-activated receptor binding protein, TRIP2, Thyroid hormone receptor associated protein 220, Thyroid hormone receptor-associated protein complex 220 kDa component, Thyroid receptor interacting protein 2, Trap220, Vitamin D receptor-interacting protein complex component DRIP205, p53 regulatory protein RB18A

• Specificity Sensitivity:

  PPAR-β (Phospho-Thr1457) antibody detects endogenous levels of PPAR-β only when phosphorylated at threonine 1457.

• References:

  Hiroshi Mano, J. Biol. Chem., Mar 2000; 275: 8126. Ken-ichiro Otsuyama, Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 5085. Liliane Michalik, J. Cell Biol., Aug 2001; 154: 799.

• All Sites:

  Human: Thr1457; Mouse: Thr1444

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human heart tissue using PPAR-β (Phospho-Thr1457) antibody. Western blot analysis of extracts from HuvEc cells, treated with Serum (20%, 30mins), using PPAR-β (Phospho-Thr1457) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using PPAR-β (Phospho-Thr1457) antibody.