• Catalog Number:

  E1A0555A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PP2A-α around the phosphorylation site of tyrosine 307 (P-D-YP-F-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P67775

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  EC 3.1.3.16, PP2A-alpha, PP2AA, RP-C, Replication protein C, Serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform

• Specificity Sensitivity:

  PP2A-α (Phospho-Tyr307) antibody detects endogenous levels of PP2A-α only when phosphorylated at tyrosine 307.

• References:

  Wen Chen, Cancer Res., Sep 2005; 65: 8183 - 8192. Kathy Bonness, Mol. Cancer Ther., Nov 2006; 5: 2727 - 2736. Stefaan Wera, J. Biol. Chem., Sep 1995; 270: 21374. Todd D. Prickett, J. Biol. Chem., Sep 2004; 279: 38912 - 38920.

• All Sites:

  Human: Tyr307; Mouse: Tyr307; Rat: Tyr307

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using PP2A-α (Phospho-Tyr307) antibody. Western blot analysis of extracts from A549 cells, using PP2A-α (Phospho-Tyr307) antibody.

P-peptide - + Immunofluorescence analysis of A549 cells, using PP2A-α (Phospho-Tyr307) antibody.