• Catalog Number:

  E1A0554A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PLK1 around the phosphorylation site of threonine 210 (K-K-TP-L-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%);Rat (Identities = 92%, Positives = 100%)

• SwissProtNo:

  P53350

• Applications:

  IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  ADRB2, EC 2.7.11.21, PLK, PLK-1, STPK13, Serine- threonine protein kinase 13, kinase PLK1

• Specificity Sensitivity:

  PLK1 (Phospho-Thr210) antibody detects endogenous levels of PLK1 only when phosphorylated at threonine 210.

• References:

  Hamanaka R., Cell Growth Differ. 5:249-257(1994). Lake R.J., Mol. Cell. Biol. 13:7793-7801(1993). Golsteyn R.M., J. Cell Sci. 107:1509-1517(1994).

• All Sites:

  Human: Thr210; Mouse: Thr210; Rat: Thr210

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PLK1 (Phospho-Thr210) antibody.