• Catalog Number:

  E1A0553A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PLCG2 around the phosphorylation site of tyrosine 1217 (L-F-L-YP-D-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P16885

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 2, EC 3.1.4.11, PLC-IV, PLC-gamma2, Phospholipase C-gamma-2

• Specificity Sensitivity:

  PLCG2 (Phospho-Tyr1217) antibody detects endogenous levels of PLCG2 only when phosphorylated at tyrosine 1217.

• References:

  Anouk Caraux, Blood, Feb 2006; 107: 994 - 1002. Yeun Ju Kim, Mol. Cell. Biol., Nov 2004; 24: 9986 - 9999. Jill C. Todt,J. Leukoc. Biol., Apr 2004; 75: 705 - 713. Marie-Pierre Gratacap, J. Biol. Chem., Sep 1998; 273: 24314.

• All Sites:

  Human: Tyr1217; Mouse: Tyr1217; Rat: Tyr1217

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human tonsil tissue using PLCG2 (Phospho-Tyr1217) antibody. Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using PLCG2 (Phospho-Tyr1217) antibody.