• Catalog Number:

  E1A0551A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PLCG1 around the phosphorylation site of tyrosine 1253 (S-R-YP-Q-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P19174

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1, EC 3.1.4.11, PLC-148, PLC-II, PLC-gamma1, PLC1, PLCG-1, Phospholipase C-gamma-1

• Specificity Sensitivity:

  PLCG1 (Phospho-Tyr1253) antibody detects endogenous levels of PLCG1 only when phosphorylated at tyrosine 1253.

• References:

  Burgess W.H., Mol. Cell. Biol. 10:4770-4777(1990). Livingston R.J., Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases. Deloukas P., Nature 414:865-871(2001).

• All Sites:

  Human: Tyr1253; Mouse: Tyr1253; Rat: Tyr1253

Western blot analysis of extracts from LOVO cells and 293 cells, treated with heat shock, using PLCG1 (Phospho-Tyr1253) antibody.